分享至
重磅活動 冷凍電鏡樣品制備前沿技術演示及研討會
長按識別二維碼
報名參與活動
特別說明：
本次活動全天直播
鑒于疫情管控因素，推薦廣大校外學員線上參加研討會
設置討論與交流環(huán)節(jié)，歡迎廣大學員積極提問
本次研討會免費
活動主辦方：
特邀報告人
劉錚
南方科技大學冷凍電鏡中心 教授
1997年取得清華大學生物物理專業(yè)博士學位，2005年在美國Wadsworth Center任研究員，2015年兼同濟大學醫(yī)學院泛血管研究所副所長，心肺血管研究所副所長，博士生導師。2020年初入職南方科技大學冷凍電鏡中心。
主要通過冷凍電子顯微學、生物化學、細胞生物學等多種技術手段研究細胞器、膜蛋白等生物大分子的工作機制。先后在國內(nèi)外重要學術期刊發(fā)表相關研究成果，其中SCI收錄40余篇。
祝建
同濟大學?教授
1982年畢業(yè)于寧夏大學農(nóng)學院，留校任教
1992—1995年蘇黎世瑞士聯(lián)邦理工學院（ETH）細胞生物所電鏡技術實驗室，中瑞聯(lián)合培養(yǎng)博士
1996—2000年上海鐵道大學醫(yī)學院
2000—2018年同濟大學生命科學與技術學院，生物電鏡技術實驗室，教授，博導
ROLAND A. FLECK
Professor of Ultrastructural Imaging, Royal Society Industry Fellow and Director of the Centre for Ultrastructural Imaging at King's College London, United Kingdom.
He is also a visiting Professor of the Faculty of Health and Medical Sciences, University of Copenhagen and Professor of the UNESCO Chair in Cryobiology, National Academy of Sciences of Ukraine, Institute for Problems of Cryobiology, Kharkiv, Ukraine. He joined King’s College London in 2013 from the National Institute for Biological Standards and Control (NIBSC), where he was head of Biological Imaging and Assay Development. At NIBSC he developed advanced imaging techniques for the control and standardisation of biological medicines and had research interests in developing differentiation protocols for myeloid leukemic and human embryonic stem cell lines as substrates for functional biological assays. He has extensive specialist knowledge of freeze fracture/freeze etch preparation of tissues and wider cryo-microscopic techniques.
As academic director of the Centre for Ultrastructural Imaging?he collaborates widely with colleagues in neuroscience and parasitology and promotes advanced three dimensional studies of cells and tissues using both room temperature and cryo electron microscopy techniques. He has extensive experience and knowledge of low temperature biology and cryopreservation having researched how cells and tissues both avoid and are damaged by chilling and freezing events. His current research interests focus on developing tools and protocols for enhancing the preservation of tissues for characterisation by electron microscopy as a capacity to enhance wider scientific collaborations.
Areas of expertise: Advanced electron microscopy techniques, cryo electron microscopy preparation techniques and electron tomography, application of serial block face and focused ion beam for the life sciences.
Jan De Bock
Jan studied biology and did his PhD in the field of olfaction, characterizing olfactory neurons in their response to odorants.
Jan has worked as a microscopy expert in different roles since 2003. He joined Leica Microsystems in 2011 as a product specialist for confocal microscopy. In 2017, he became member of the newly formed Workflow and Application Team responsible for correlative workflows, in particular involving sample preparation and imaging under cryogenic conditions.
Abstract
Cryo Electron Microscopy workflows are a state-of-the-art tool to investigate proteins in the cellular context with subnanometer resolution. To succeed in this, light microscopes performing under cryogenic conditions are essential for an early quality check and to identify target structures for the subsequent EM analysis.
In this webinar we show Leica Microsystem′s cryo microscope solutions for the assessement of the sample quality, a safe sample transfer and ?super resolution imaging under cryogenic conditions as a basis for precise targeting.
冷凍電子斷層圖像。與核孔復合體相連的蛋白酶體（紫色）。由分子結構系Ben Engel博士提供。生物學，生物化學MPI，馬丁斯利德，德國
Leica EM Cryo CLEM
細胞內(nèi)大分子原位結構研究
徠卡顯微系統(tǒng)可為不同品牌SEM-FIB雙束顯微鏡定制提供完全集成的低溫電子斷層成像工作流程，以滿足研究需要。
了解更多：徠卡顯微
