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DNA連接與轉(zhuǎn)化 快速細(xì)胞破碎法實(shí)驗(yàn)步驟 挑取轉(zhuǎn)化平板上的單菌接種于含相應(yīng)抗生素的2ml LB中,37℃振蕩培養(yǎng)至A590值為0

來源：互聯(lián)網(wǎng) 關(guān)鍵詞：Immunochemistry Protocols Western-blot Antibody

ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall:???? ????????165 x 130 mmMedium: ????????165 x 200 mmLarge:??? ????????165 x 260 mm5% Analytical GelsReagent1 mm Small1 mm Medium1 mm Large10X TBE Buffer (ml)33

來源：互聯(lián)網(wǎng) 關(guān)鍵詞：融合標(biāo)簽 蛋白酶 酶切位點(diǎn)

Protocol for Protein Extraction 10 % w/v TCA/ acetone/ 0

來源：互聯(lián)網(wǎng) 關(guān)鍵詞：Western Blot 實(shí)驗(yàn)試劑

Run protein gel

來源：互聯(lián)網(wǎng) 關(guān)鍵詞：Far-Western Blotting

Small scale His-Tag fusion protein purification under denaturative conditions ? Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates, usually known as inclusion bodies (IB)

來源：互聯(lián)網(wǎng) 關(guān)鍵詞：Western Blot