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化學(xué)生物學(xué)實(shí)驗(yàn)技術(shù)>Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
關(guān)鍵詞： enzymatic characterization來源： 互聯(lián)網(wǎng)
The dynamic addition of O -GlcNAc to target proteins is now recognized as a major signaling paradigm impacting phosphorylation, protein turnover, gene expression, and other posttranslational modifications influencing epigenetics. Here we describe the production of and methods for assay of the recombinant enzymes of O -GlcNAc cycling: O -linked GlcNAc Transferase (OGT) and O -GlcNAcase (OGA).
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