A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function
Cancer gene profiling has greatly profited from the progress in high-throughput technologies including microarray-, sequencing-, and bioinformatics-based methods. The flood of data generated during the last decade has provoked a panel of “-omics” fields that significantly changed our understanding of malignant diseases. However, while the terms “-omics” and “-ome” in principle refer to the completeness of a genetic approach, we are in fact far from a complete understanding of cancer progression. We may understand gene expression patterns better and successfully use gene signatures for outcome prediction and prognosis, but truly promising molecular targets still have to find their way into novel therapeutic concepts. In this chapter, we will show how more comprehensive strategies, integrating multiple layers of genetic information, might in the future provide a more profound functional understanding of cancer.
- Identification of Altered Gene Expression Associated with Pigmentary Lesions by Differential Display Analysis
- Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivity
- Circumventing Tumor Resistance to Chemotherapy by Nanotechnology
- Isolation and Culture of Human Brain Tumor Cells
- Complete Array of HOX Gene Expression by RQ-PCR
- Quantitative PCR Detection of c-erbB-2 Gene Amplification
- Nanoshells for Photothermal Cancer Therapy
- 7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy
- Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
- Application of Silicon Sensor Technologies to Tumor Tissue In Vitro: Detection of Metabolic Correlates of Chemosensitivity