Poor cell culture practice leads to poor science due largely to issues of cross-contamination between cell lines and of microbial contamination, but can be avoided by careful quality control and good laboratory practice. This chapter provides a brief and practical outline of the steps needed to mitigate the risks associated with poor cell culture practice. Good Laboratory Practice (GLP) is a set of principles that provides a framework within which laboratory studies are planned, performed, monitored, recorded, reported, and archived to ensure the reliability of data generated within a compliant laboratory. A key feature of this is the generation of quality-control methods and data management within the cell culture laboratory.