精品国产亚洲一区二区三区大结局,日韩国产码高清综合,国产精品丝袜综合区另类,久久午夜无码午夜精品

實驗方法> 細胞技術> 前沿科技及其它>MTT Growth Assays in Ovarian Cancer

MTT Growth Assays in Ovarian Cancer

關鍵詞: mtt growth assays來源: 互聯(lián)網

The MTT (3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide) growth assay developed by Mosmann (1 ) offers a simple, rapid, and precise measurement of cell viability and proliferation of adherent cell lines (2 ). The value of this assay is in the screening of large numbers of samples. The MTT assay, a quantitative colorimetric assay is based on the living cell’s ability to reduce the tetrazolium salt MTT, a pale yellow substrate to a dark-blue formazan product. The mitochondrial succinate-dehydrogenases (3 ) of viable cells cleave the tetrazolium ring in active mitochondria into formazan crystals. The crystals can be dissolved in acid isopropyl alcohol, mineral oil (4 ), or dimethyl sulfoxide (DMSO) (5 ). The resulting blue solution can be measured semiautomatically using a scanning multiwell spectrophotometer. Our laboratory has successfully applied the MTT-based growth assay with some modifications (6 ) to investigate the growth effects of human cytokines on HOC cell lines (7 ), but we have to keep its limitations and pitfalls in mind (see Note 1 ). For use in tests of floating cell lines, the MTT assay may be less optimal. Using this assay for screening of primary tumor samples may produce limited results, because cell contaminants may result in high-background values (4 ). However, accepting the limitations of the MTT assay, the optimum assay conditions have to be selected and adapted to the cell lines that are under investigation. The MTT-based growth assay, as described in this chapter, is a reliable and sensitive test for the determination of cell growth of human ovarian-carcinoma cells.

推薦方法

Copyright ?2007 ANTPedia, All Rights Reserved

京ICP備07018254號 京公網安備1101085018 電信與信息服務業(yè)務經營許可證:京ICP證110310號

97超级免费视频在线观看| 久久综合娱乐中文网| AV天堂手机福利网| 亚洲二亚洲欧美一区vr| 裸体午夜一级视频| 日本亚洲欧洲一区二区| 国产高清乱码女大生AV| 下载风骚美女想吃大机吧| 日本 视频 一区二区| 天天日天天干天天天天操| 国产精品粉嫩懂色av| 美女主播被操流水| 日韩欧美一级特黄大片欧| 日韩精品诱惑一区?区三区| 欧亚洲嫩模精品一区三区| 欧美日本大白屁股大黑逼操逼视频| 那种视频在线观看你懂的| 日韩欧美一区二区三区在线视频| 鸡巴插进缝里 日本| 国产女主播喷出白浆视频| 日韩在线视频不卡一区二区三区| 亚洲综合一区国产精品| 抽插肥嫩小穴的视频| 午夜福利国产三级片| 啊好爽好多水深插射视频| 男人把昆吧放女人屁股里| 校花内射国产麻豆欧美一区| 一区二区国产精品免费视频| 精品久久av免费一区二区三区| 精品无码国产一区二区三区麻豆| 91成人精品国语自产拍| 日韩激情视频在线看免费| 三级片成人京东热五月天| 日韩午夜免费av在线| 99久久99久久精品视频| 国产欧美一区二区精品久久久| 精品一区二区视频在线观看| 一区二区三区av精品| 国产精品白浆一区二区三区| 日韩乱码一区二区三区中文字幕| 欧美一区二区三区刘玥|