G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. DNA G-quadruplexes have recently emerged as attractive cancer therapeutic targets. It has been shown that the 3′ G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy has been shown to be a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR methodology used in our research group for structure determination of G-quadruplexes in solution and their interactions with small molecule compounds. An example of a G-quadruplex structure formed in the human telomere sequence recently solved in our laboratory is used as an example.
- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA
- Protein Detection Using Reversible Metal Chelate Stains
- 圓形濾紙色譜法分離氨基酸
- 散在重復(fù)序列
- Study of GPCRProtein Interactions by BRET
- In-gel digestion of proteins for peptide fingerprint mapping
- 新疆葡萄干
- Glycosyltransferases in Chemo-enzymatic Synthesis of Oligosaccharides
- SH2 Domain-Based Tyrosine Phosphorylation Array
- 生物微核對(duì)環(huán)境污染的指示