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實(shí)驗(yàn)方法> 生物化學(xué)技術(shù)> 化學(xué)生物學(xué)實(shí)驗(yàn)技術(shù)>G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

關(guān)鍵詞: structures g-quadruplex-interactive來(lái)源: 互聯(lián)網(wǎng)

G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. DNA G-quadruplexes have recently emerged as attractive cancer therapeutic targets. It has been shown that the 3′ G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy has been shown to be a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR methodology used in our research group for structure determination of G-quadruplexes in solution and their interactions with small molecule compounds. An example of a G-quadruplex structure formed in the human telomere sequence recently solved in our laboratory is used as an example.

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